In this experiment, all antagonistic fungal isolates were cultured
separately on Petri plates containing PDA medium and incubated at
25 C for 48e72 h. After this time, a one-cm- diameter plug of
R. solani was cultured on separate Petri plates containing PDA
culture medium and these two groups of plates were placed on
each other inversely after removing their lids. Petri plates were
then incubated at 25 C. After two weeks, the diameter of R. solani
colonies was measured in different plates and growth inhibition
percentages were determined using the following formula: