Bacterial suspension of E. coli, A. hydrophila and Y.
enterocolitica was used for sensitivity testing. Bacterial
suspension was prepared by inoculating pure colonies in
0.1% peptone water and adjusting OD = 0.5 units at
610 nm. The bacterial suspensions were serially diluted.
Sterile, deionized distilled water was spiked by either of
dilution of respective bacterial suspension of all test cultures.
BWTK was inoculated with 15 ml of spiked, sterile,
deionized distilled water. It was then incubated at
37°C for 12 h. PCR was performed from the samples
drawn from positive BWTK.