Consumption of omega-3 fatty acids (x-3’s), whether from fish oils, flax or supplements, can protect
against cardiovascular disease. Finding plant-based sources of the essential x-3’s could provide a sustainable,
renewable and inexpensive source of x-3’s, compared to fish oils. Our objective was to develop a
rapid test to characterize and detect adulteration in sacha inchi oils, a Peruvian seed containing higher
levels of x-3’s in comparison to other oleaginous seeds. A temperature-controlled ZnSe ATR mid-infrared
benchtop and diamond ATR mid-infrared portable handheld spectrometers were used to characterize
sacha inchi oil and evaluate its oxidative stability compared to commercial oils. A soft independent model
of class analogy (SIMCA) and partial least squares regression (PLSR) analyzed the spectral data. Fatty acid
profiles showed that sacha inchi oil (44% linolenic acid) had levels of PUFA similar to those of flax oils.
PLSR showed good correlation coefficients (R2 > 0.9) between reference tests and spectra from infrared
devices, allowing for rapid determination of fatty acid composition and prediction of oxidative stability.
Oils formed distinct clusters, allowing the evaluation of commercial sacha inchi oils from Peruvian markets
and showed some prevalence of adulteration. Determining oil adulteration and quality parameters,
by using the ATR-MIR portable handheld spectrometer, allowed for portability and ease-of-use, making it
a great alternative to traditional testing methods.