Magnetic cell separation. The isolation of NK cells was done using human CD56+CD8+/CD8ˉ NK cell
Isolation Kit from Miltenyi Biotec, according to manufacturer’s protocol; with a slight modification.
Briefly, the lymphocytes was isolated from human PBMCs, and only the lymphocytes were magnetically
labeled with a cocktail of biotin-conjugated antibodies against lineage-specific antigens and a cocktail of
MicroBeads.