and pretreated EFB
were determined by using standard Biomass Analytical Procedures provided by National Renewable
Energy Laboratory (NREL) [3]. The sugars content that are glucose and xylose in the hydrolysate, and
ethanol concentrations were analyzed by using High Performance Liquid Chromatograph (HPLC). The
HPLC (Waters, USA) system was equiped with AMINEX HPX 87H column, and a guard column, an
automated sampler, a gradient pump, and a refractive index detector. The mobile phase was 5mM H2SO4
at flow rate of 0.6 mL min-1 and oven temperature was maintained at 65oC. Prior to HPLC injection, all
samples (derived from solids and hydrolysate) were neutrilized with calcium carbonate, shake for 2 min,
and filtered through 0.2 m syringe filters.