3. Results and discussion
Boar spermatozoa have the highest sensitivity
to cold shock, among all the domestic
animals, a sensitivity that could be caused by
the plasma membrane special composition, in
phospholipids and cholesterol [4]. As boar
spermatozoa can acquire resistance to cold shock,
if incubated at low extension rates, for 1-5 h at
ambient temperatures [5, 6], a 4h equilibration
time of the sperm was applied before