A noticeable diversity was highlighted in the ability of
L. casei, L.
paracasei and L. rhamnosus
strains to withstand different harsh
growth conditions, suggesting that different stress factors should
be applied to identify the most promising candidates for food
applications.
In this investigation 13 strains, capable to survive to stress
conditions as high acidity, bile salt and high osmolarity, ful
fi
l all the
pre-requisites for probiotic use. Most of these strains were isolated
from human faeces and wine, matrices that should be considered
for the isolation of new strains with desired technological proper-
ties. Until now, in fact, the strains belonging to
L. casei, L. paracasei
and
L. rhamnosus
species for which bene
fi
cial effects were clinically
assessed and that are commercially available, were isolated mainly
from fermented milk, cheese and fermented vegetables.
Finally, this study can represent a basis for undertaking in-
vestigations on physiological traits to be de
fi
ned at the molecular
level in order to identify appropriate genetic markers and methods
for the rapid characterization of strains
A noticeable diversity was highlighted in the ability of
L. casei, L.
paracasei and L. rhamnosus
strains to withstand different harsh
growth conditions, suggesting that different stress factors should
be applied to identify the most promising candidates for food
applications.
In this investigation 13 strains, capable to survive to stress
conditions as high acidity, bile salt and high osmolarity, ful
fi
l all the
pre-requisites for probiotic use. Most of these strains were isolated
from human faeces and wine, matrices that should be considered
for the isolation of new strains with desired technological proper-
ties. Until now, in fact, the strains belonging to
L. casei, L. paracasei
and
L. rhamnosus
species for which bene
fi
cial effects were clinically
assessed and that are commercially available, were isolated mainly
from fermented milk, cheese and fermented vegetables.
Finally, this study can represent a basis for undertaking in-
vestigations on physiological traits to be de
fi
ned at the molecular
level in order to identify appropriate genetic markers and methods
for the rapid characterization of strains
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