oiler eggs were from a single 45-wk-old commercial broiler breeder flock and were held for 2 D under standard storage conditions prior to being set. 2,400total eggs. which was comprised of 3 injection treatments (IT) and 2 IAN, for a total of 6 treatment groups. The IT were designated as: noninjected control (NIC), diluent-injected control (DIC), and EM1 vaccine-injected (VI)