A used for cultivating mushroom improved matrix, it is substantially free of cellulose, includes a large number of grain and micro-nutrient add-ons, the grain through the boiling part of the sterilization, in order to kill bacteria, through the cooling in order to induce heat-resistant spore germination, and in the germination of the spores mature enough to produce new spore the steam is antiseptic before, the substrate can be used for the growth of fungi, include mushroom, especially mushroom.