The polymorphism of A781G locus of the growth hormone (GN) gene was analyzed in 112 Vembur sheep. Haelll-RFLP of the GN gene (422 bp) existence of two genotype viz., AA (366 and 56 bp) and AB (422, 366 and 56 bp),and two alleles viz., A and B . Genotypic frequencies for AA and AB were 0.71 and 0.29 for A and B respectively. BB genotype was absent.Highly significant Chi-square value (P