One gram of flesh tissue was ground with 5 ml ethanol and the mixture was centrifuged at 12,000 × g for 10 min at 4◦C. After that, 0.1 ml of ethanol extract was mixed with 1 ml of 2 g/L anthrone in 706 g/L H2SO4. The mixture was incubated at 100◦C for 15 min, cooled in a water bath and the total sugar content was measured absorbance at 625 nm