Phosphate concentration
Influence of phosphate on cell growth and CPT production
was studied in detail, and the results are presented in Fig. 5.
1/2 strength MS medium containing 1.25 mM phosphate produced
good cell growth (12.84 ± 1.1 g/flask FW) compared
with other concentrations. When the concentration of phosphate was increased to 2.5 mM, cell growth reduced.
Similar CPT yield was recorded in 0.3125, 0.625 and
1.25 mM of phosphate. An increase in the phosphate concentration
above 0.3125 mM did not have an impact on enhancing
CPT production. The increase in phosphate concentration
above the optimum level had a negative effect on both cell
growth and CPT production.