Bacteriophages incorporated into acetate film caused visual
changes in the film and changes in its tensile properties. The
thickness, elongation and puncture resistance were not altered
with the addition of bacteriophages.
The antimicrobial activity of the film was shown in both the
diffusion method in liquid medium and in the method of solid
medium diffusion evidenced by the growth curve and the inhibition
formed, respectively, when incubated at 35 C.
Bacteriophages remained viable for 14 days of evaluation. After
that time, they were no longer detected in the film. The Atomic
Force Micrograph showed a change in the morphological structure
of the surface of the films with the addition of bacteriophages as
compared to control.
The techniques of incorporation of bacteriophages into film
need to be improved so that they remain viable for longer in the
packaging and can be used in future refrigerated food storage and
consumption.