Methods: A range of rational methods were applied in determining specific carbohydrate constituents:RS, NSP and RO, including fructans. For comparison, empirical methods AOAC 991.43 (2001.03) and AOAC 2009.01 were applied, based on determination of gravimetric residues for high molecular weight and size-exclusion HPLC analysis of the ethanol filtrate for low molecular weight components.