In order to amplify the ITS region
the primers Diplost_F3
(50- AGGAATTCCTGGTAAGTGCAAGe30)
/Diplost_R4 (50- TATGCTTAA
ATTCAGCGGGTe30) were used
and to amplify the mt DNA-Cox1 region the
primers COI2575F (50-TTTTTTGGGCATCCTGAGGTTTAT-30)/COI302
1R (50- TAAAGAAAGAACATAATGAAAATGe30) were used (Bowles,
Blair, & McManus, 1992).