Carrageenan-Induced Granuloma Pouch Model [104].
Carrageenan-induced granuloma pouch model is an excellent
subacute inflammatory model in which fluid extravasations,
leukocyte migration, and various biochemical exudates
involved in inflammatory response can be detected readily.
The air pouch has the advantage of supplying a suitable space
for the induction of inflammatory responses. The injection
of irritants such as carrageenan into subcutaneous air pouch
on the dorsal surface of rats initiates an inflammatory
process. Procedure. The animals used in this method are
rats divided into five groups (n = 6), fasted overnight, and
allowed free access to water. The animals are administered
with vehicle, standard drug, and test drug. One hour after
dosing, the back of the animal is shaved and disinfected. With
a very thin needle, subcutaneous dorsal granuloma pouch
is made in ether anaesthetized rats by injecting 6 mL of air,
followed by injection of 4 mL of 2% carrageenan in normal
saline to avoid any leakage of air and the treatment continued
for seven consecutive days. On day 8, the pouch is opened
under anesthesia and the amount of exudates was collected
with a syringe. The average volume of exudates, total WBC
count, and weights of granuloma is determined.