Briefly, to each pre-treated sample, 860 mL of pre-heated at 60 C TNE extraction buffer
(10 mM Tris, 150 mM NaCl, 2 mM EDTA, 1% SDS), 100 mL of 5 M guanidine hydrochloride solution and 40 mL proteinase K solution (20 mg mL1) were added and the mixturewas vortex stirred.