To produce wheat deletion lines and translocation lines for cytogenetic studies or breeding, a cross was made between the “Chinese
Spring”-gametocidal chromosome 2C disomic addition line (CS-2C′′) and the “Chinese Spring”-Lophopyrum elongatum 6E disomic
addition line (CS-6E′′). Meiosis of F1
pollen mother cells (PMCs) was analyzed. F2 plants derived from self-crossing of F1
hybrids
were subjected to chromosome C-banding and genomic in situ hybridization (GISH) to detect chromosome variations. The results
showed that large quantities of abnormal chromosomes in F1
PMCs were observed and 9 translocation plants and 10 wheat deletion
plants were characterized among 95 progeny plants of F2
. There were seven wheat-L. elongatum translocation plants and two
wheat intergenomic translocations in nine translocation plants. The translocation frequency was 7.37 % and the deletion frequency
was 10.5 %. These results indicate that the induction of chromosomal variations using gametocidal chromosome 2C was efficient.
Translocations with small alien segments and deletions with minimal loss of wheat chromatin were preferable for breeding purposes.