The relative molecular mass of the native enzyme was
measured by high performance liquid chromatography on
a Protein KW-803 Shodex Gel Filtration column
(8 mm · 300 mm). Sodium dodecyl sulfate-polyacrylamide
gel electrophoresis (SDS-PAGE) (10% polyacrylamide)
was performed by the procedure of Laemmli (Laemmli,
1970)