characterized for nutrients (NH4
+, NO3
-
, Total Kjeldahl
Nitrogen [TKN] and total phosphorus), pH, and
indicator organism (Escherichia coli). All chemical
analyzes were performed in the Laboratory of Water
Chemistry of Besancon and microbiological parameters
were tested by the Laboratory of the Hospital of
Besancon. All these analyzes were conducted
according to standards methods. These laboratories
are accredited according to the ISO 17025 norm. Input
and output effluent samples were analyzed for pH using
a pH meter NF T 90 008. Chemical Oxygen Demand
(COD) was determined by colorimetric method using
potassium dichromate ISO 6060:1989. Biochemical
Oxygen Demand (BOD7
) was measured by the dilution
and seeding method with allylthiourea addition
according to ISO 5815-2:2003. BOD7
was shown over
BOD5
for timing reason for laboratory since sampling
was done on Monday and analysis on the next
Monday. The total phosphorous was determined by
inductively coupled plasma mass spectrometry
according to standard methods ISO 118885, Ammonia
content was determined by colorimetric method NFT
90-015-1, TKN by EN 25663 and nitrate content by Ionic
Chromatography according to the standard method
ISO 10304-1. Escherichia coli concentration was
determined by using a standard method ISO 9308-1
and total floral by ISO 6222:1999. The removal
efficiency (%) of S, SP and P were calculated, using
the following equations: