Recently, interest in developing pineapple wine, one of the local pineapple products, has increased as a potential product for exportation. However, the production process specific to pineapple wine manufacture that is required to improve and standardize the quality, consistency and varieties of pineapple wine, is still very much at the initial stage of development. Autochthonous yeasts are reported to be wild yeasts that produce the unique flavours and exceptional quality when used for traditional wine fermentation [1].