Acclimatization conditions
For the acclimatization, rooted tissue culture regenerants (control and CK-treated) were washed and potted in 12.5 cm pots volume containing sand, soil, vermiculite (1:1:1, v/v) treated with 1% antifungal compound. The plantlets were maintained for 1 month in the mist house with day/night temperatures of 30/12 ◦C, relative humidity between 80–90%, a misting interval of 15 min every 10 s. The plantlets were then transferred to a greenhouse with 30–40% relative humidity, day/night temperatures of 30/15 ◦C with an average PPFD of 450 mol m−2 s−1. During the experiment, photoperiod
was that of prevailing natural conditions during summer (12 h). After 2 months, the greenhouse acclimatized plants were
harvested.
Acclimatization conditionsFor the acclimatization, rooted tissue culture regenerants (control and CK-treated) were washed and potted in 12.5 cm pots volume containing sand, soil, vermiculite (1:1:1, v/v) treated with 1% antifungal compound. The plantlets were maintained for 1 month in the mist house with day/night temperatures of 30/12 ◦C, relative humidity between 80–90%, a misting interval of 15 min every 10 s. The plantlets were then transferred to a greenhouse with 30–40% relative humidity, day/night temperatures of 30/15 ◦C with an average PPFD of 450 mol m−2 s−1. During the experiment, photoperiodwas that of prevailing natural conditions during summer (12 h). After 2 months, the greenhouse acclimatized plants wereharvested.
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