This study evaluates the effects of partial vacuum storage on longevity and anti-oxidative responses of primed
bitter gourd (Momordica charantia L.) seeds. Priming was achieved by mixing the seeds with moist vermiculite 25 °C
for 36 h, followed by air-drying to the original moisture level. Primed seeds were vacuum-packed and stored at 25 °C for
up to 12 months. Priming improved seed germination, reduced lipid peroxidation and enhanced anti-oxidative activity
prior to storage. However, primed seeds accumulated more total peroxide than non-primed control after 12 months non-
vacuum storage, and this led to a marked decrease in seed longevity. Increased total peroxide levels were associated with
decreased percentage of 2,2-azinobis(3-ethylbenzothiazoline-6-sulfonate) (ABTS) radical inhibition. Partial vacuum
storage proved useful in extending the longevity of primed seeds for up to 12 months. Improved longevity was related to
enhanced anti-oxidative activity that minimized the accumulation of total peroxide during long-term storage.