effect of a combination of mono- and disaccharides is
unknown.
Growth regulators have a low importance for
germination, but are important for the subsequent
development of protocorms (Stewart 1989, De Pauw
et al. 1995). Their influence often depends on the
concentration and the orchid species (Van Waes and
Debergh 1986), e.g. IAA inhibited germination of
Dactylorhiza purpurella while kinetin inhibited
formation of protocorm rhizoids (Hadley 1970).
The objective of this work was to determine the
influence of sugars and growth regulators on shoot and
root growth rate and length of Dactylorhiza seedlings.
We used mature seeds of Dactylorhiza majalis
(Reichenb.) Hunt et Summerh., Dactylorhiza incarnata
(L.) Soó ssp. incarnata, Dactylorhiza incarnata (L.) Soó
ssp. ochroleuca, Dactylorhiza maculata (L.) Soó ssp.
maculata. All these taxa are weakly or medium
mycotrophic (Michl 1981). Mature capsules were
collected during July in Southern Bohemia. The seeds