In this study, enhanced recovery of phenolic compounds from bitter melon (Momordica charantia) was achieved by subcritical water extraction (SWE). The extraction was carried out at a constant pressure of 10MPa and the effects of extraction temperature (130–200°C) and water flow rate (2–5ml/min) were determined on the amount of phenolic compounds and the most suitable extraction condition was found to be at the temperature between 150 and 200°C and at the flow rate of 2ml/min. The amount of phenolic compounds in the extracts obtained by SWE (52.63mg gallic acid equivalents (GAE)/g dry weight (DW)) was significantly higher than those in the extracts obtained by MeOH extraction (5.00–6.00mg GAE/gDW) and soxhlet water extraction (5.00–7.00mg GAE/gDW). The most phenolic acids contained in bitter melon were catechin, whose content in the extracts obtained by the SWE, MeOH extraction, and soxhlet extraction were 46.16, 1.61, and 1.77mg/gDW, respectively. Although the amount of phenolic compounds in the extracts obtained by SWE at lower temperature was found to be lower than those obtained at higher temperature, they exhibited higher antioxidant activity. Nevertheless, all extracts obtained with SWE had approximately threefold higher antioxidant activity than those obtained by extraction with MeOH or water at ambient pressure conditions.
Enhanced recovery of phenolic compounds from bitter melon ( Momordica charantia) by subcritical water extraction. Available from: http://www.researchgate.net/publication/244308150_Enhanced_recovery_of_phenolic_compounds_from_bitter_melon_(_Momordica_charantia)_by_subcritical_water_extraction [accessed Nov 3, 2015].
In this study, enhanced recovery of phenolic compounds from bitter melon (Momordica charantia) was achieved by subcritical water extraction (SWE). The extraction was carried out at a constant pressure of 10MPa and the effects of extraction temperature (130–200°C) and water flow rate (2–5ml/min) were determined on the amount of phenolic compounds and the most suitable extraction condition was found to be at the temperature between 150 and 200°C and at the flow rate of 2ml/min. The amount of phenolic compounds in the extracts obtained by SWE (52.63mg gallic acid equivalents (GAE)/g dry weight (DW)) was significantly higher than those in the extracts obtained by MeOH extraction (5.00–6.00mg GAE/gDW) and soxhlet water extraction (5.00–7.00mg GAE/gDW). The most phenolic acids contained in bitter melon were catechin, whose content in the extracts obtained by the SWE, MeOH extraction, and soxhlet extraction were 46.16, 1.61, and 1.77mg/gDW, respectively. Although the amount of phenolic compounds in the extracts obtained by SWE at lower temperature was found to be lower than those obtained at higher temperature, they exhibited higher antioxidant activity. Nevertheless, all extracts obtained with SWE had approximately threefold higher antioxidant activity than those obtained by extraction with MeOH or water at ambient pressure conditions. ขั้นสูงกู้คืนม่อฮ่อมจากมะระ (สมุนไพรมะระขี้นก) โดยจัดสรรน้ำ subcritical จาก: _by_subcritical_water_extraction _ (_Momordica_charantia) http://www.researchgate.net/publication/244308150_Enhanced_recovery_of_phenolic_compounds_from_bitter_melon [เข้าถึง 3 nov, 2015]
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