Solution(1-2 ml.) containing metal ions (0- 50 Mg) was transferred to separating funnel and reagent solution (1 mL ; 0.2% w/v in methanol) and appropriate buffer solution (2 mL) were added. To this chloroform (5 mL) added was mixed well. The layers were allowed to separate and organic layer was collected in 10 mL volumetric flask. The extraction was repeated with chloroform (3 mL) The final volume was adjusted with chloroform.