A novel method for the extraction of milk fat using dichloromethane (CH2Cl2)–ethanol was compared to
the Rose-Gottlieb (IDF Standard 1D, 1996) extraction procedure. The new extraction method consisted of
a fast (approximately 30 min for 20 samples) and simple procedure involving direct mixing of raw cow
milk samples with a dichloromethane–ethanol solution (2/1, v/v).