Gram staining
Preparation of Nutrient broth solution:
Nutrient broth is a media which helps to grow bacteria with the necessary ingredients in a broth for the analysis of bacteria. A broth is prepared with the following ingredients.
Water -
100ml
Peptone -
0.2 gm
Yeast -
0.2 gm
Beef extract -
0.3 gm
NaCL -
0.5 gm
The solution is sterilized for 15 min at 1200C and it is allowed to cool. Then the fabric is immersed in the broth solution for 24 hours.