kinless chicken breast samples were examined for levels of
total aerobic bacteria, Pseudomonas spp., Enterobacteriaceae and Brochothrix thermosphacta. Duplicate samples from each treatment
were aseptically opened on the sampling days; then a 25 g portion
of skinless chicken breast was aseptically transferred to a sterile
stomacher bag. Next, 225 mL of 0.1 M sterile sodium phosphate
buffer solution (pH 7.0) was added and homogenized for 1 min by
a Stomachers 400 laboratory blender (Seward, UK). A series of
decimal dilutions was carried out according to recommended
microbiological protocols