The regeneration potential from the nodal segments
of Morus nigraL as observed in present work was
attention creating. On MS medium as control, explants
showed no response for a couple of weeks. MS medium
supplemented with BAP (5.0 mg/l), influenced shoot
proliferation from the nodal explants of mature trees
(Fig. 1). Similar results have been achieved by in case of
various mulberry species from nodal segments on MS
medium supplemented with different concentrations of
BAP and NAA.
These In vitro raised shoots when subcultured
in reduced concentration of BAP (1.5 mg/l),
sprouted and multiple shoot proliferation was observed in
4-5 days of inoculation. The leaves produced by In vitro
raised shoots were similar to those of mother plant
(Fig. 2).
Same results were obtained by Hussainet al.[19]
from nodal explants of Morus laevigata. The production
of shoots from nodal segments was also established
by Aniset al.[23]. But these workers achieved shoot
multiplication by culturing explants on MS medium
fortified with BAP (2.5 mg/l) along with Glutamin (1 mg/l).
This medium facilitated the elongation of shoots and
sprouting of nodal segments from In vitro grown micro
cuttings.
NAA (0.1-0.5 mg/l) was found to be the optimum
concentration for the production of roots in the In vitro
raised shoots (Fig. 3). Same results have been reported by
Ohyama and Oka [24], while studying effect of NAA on
rooting from Morus alba,Anis,et al.[23] also achieved
80% rooting in case of shoots cultured on MS medium
supplemented with NAA (1.0 mg/l).
The regeneration potential from the nodal segmentsof Morus nigraL as observed in present work wasattention creating. On MS medium as control, explantsshowed no response for a couple of weeks. MS mediumsupplemented with BAP (5.0 mg/l), influenced shootproliferation from the nodal explants of mature trees(Fig. 1). Similar results have been achieved by in case ofvarious mulberry species from nodal segments on MSmedium supplemented with different concentrations ofBAP and NAA.These In vitro raised shoots when subculturedin reduced concentration of BAP (1.5 mg/l),sprouted and multiple shoot proliferation was observed in4-5 days of inoculation. The leaves produced by In vitroraised shoots were similar to those of mother plant(Fig. 2).Same results were obtained by Hussainet al.[19]from nodal explants of Morus laevigata. The productionof shoots from nodal segments was also establishedby Aniset al.[23]. But these workers achieved shootmultiplication by culturing explants on MS mediumfortified with BAP (2.5 mg/l) along with Glutamin (1 mg/l).This medium facilitated the elongation of shoots andsprouting of nodal segments from In vitro grown microcuttings.NAA (0.1-0.5 mg/l) was found to be the optimumconcentration for the production of roots in the In vitroraised shoots (Fig. 3). Same results have been reported byOhyama and Oka [24], while studying effect of NAA onrooting from Morus alba,Anis,et al.[23] also achieved80% rooting in case of shoots cultured on MS mediumsupplemented with NAA (1.0 mg/l).
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