bacteriaRumen digesta (5 g) for enumeration of cellulolytic and pro-teolytic bacteria were collected on day 7 of each period before morning feeding. The anaerobic techniques of Hungate as modifiedby Bryant were used for the growth of organisms and preparationof media. For cellulolytic bacteria, Hungate tubes containing media andfilter paper as the sole source of carbohydrate for grow-ing cellulolytic bacteria and Hungate tubes containing media and gelatinpowder as the protein source was prepared. Then, rumen fluid was dilutedwith anaerobic dilution solution and inoculated to the tubes. Cultureswere grown at 39◦C for 14 days. Cellulolytic and proteolytic bacteria werealso enumerated in both medium using the MPN procedure described byDehority et al. (1989). Degradation of filter paper and gelatin liquefactionwere considered as the presence of cellulolytic and proteolytic bacteria,respectively.