This cell suspension was diluted
100 times to obtain 106 CFU/mL before use. The compounds were
dissolved in DMSO and then added to bacteria suspension to obtain the
final concentration of 5% (v/v) DMSO or less. Serial twofold dilutions
from 200 μg/mL of the compounds were performed in 96-well microtiter
plates. Each well contained 100 μL of sample of each concentration.
Into each well was then introduced 100 μL of the bacterial suspension.
The final concentration range of the test compounds was 100–0.781
μg/mL, and the plates were incubated at 37 °C for 24 h