Tissue culture studies on chrysanthemum were first initiated by Morel & Martin in 1952. They used meristem tip culture to obtain virus free plants. Now tissue culture is a necessary first step in the production of virus indexed chrysanthemums. It serves as a nuclear stock for the delivery of high quality cuttings (Debergh, 1994). Much work has been done on callogenesisand and organogensis of chrysanthemums. Many workers have obtained shoot organogenesis form floret derived callus. Sutter & Langhans (1981) obtained adventitious shoots from callus derived from leaf and shoot tips.