The operation of soft-shell crawfish culture is based upon crawfish molting behavior. Mean molt interval and molting percentage are the main factors determining the production rate of a culture facility. Effects of temperature on mean molt interval and molting percentage of red swamp crawfish (Procambarus clarkii) were investigated in laboratory-scale recirculating culture system in two seasons. In this study, higher temperatures resulted in shorter molt intervals. Corresponding to temperatures ranging from 15 to 30 °C, with a 3 °C increment, the molt interval decreased non-linearly from 71.2 to 22.6 days. However, lower temperatures seemed to mitigate crawfish mortality and resulted in higher molt percentages. Additionally, the major portion of the mortality occurred within the first 2 weeks of the tests. The results of this study provide a quantitative basis for recommending proper temperatures for a soft-shell crawfish operation. From the production perspective, the ideal temperature lies at the high end of the test temperature range.