Nevertheless, the different sensitivities exhibited by the real time kits can have considerable clinical impact. For instance, as the viralRNA load decreases during the recovery the various real-time RT-PCR assays may yield undetectable resu at fferent time points, potentially resulting in isolation time. This issue was exemplified during the management of an EVD infected patient in our center, as we obtained different real-time RT-PCR results with t Roche and Altona assays run on the same samples during the convalescent 194 However, one can argue that when a patient shows signs of clinical cure, the low-level detection of viral RNA proba bly reflects genomic remnants rather than residual infectious virus 24l Therefore, Ct values obtained during the recovery phase should probably not be overemphasized.