was identified by retention time and spiking the sample with
a standard solution of c-oryzanol from bran rice (Cymit, Spain) and
the purity of peaks was confirmed by comparison of the spectra
and by MS analysis. c-Oryzanol content was quantified by percentage
of peak area according to the calibration curve prepared with
c-oryzanol standard solutions. Replicates were independently analysed
and results were expressed in mg c-oryzanol/100 g.