The antagonistic activities of soil alone (1 g), saliva alone (1 ml) and the mixture of both (1 g soil and 1 ml saliva) were tested against the pathogenic microbes.
The method described by Umechuruba and Nwachukwa (1997) was used for testing the antimicrobial activities of the cell free culture media of both soil bacteria and fungi on the growth of the tested pathogenic microbes.
The pathogenic fungus and bacteria were treated with different concentrations of fungal and bacterial filtrates of the mixture (soil + saliva).
The dry weight of the pathogenic microbes was determined as mg according to the method of Bouknight and Sadoff (1975).
The antagonistic effects of the bacterial and fungal species isolated from the mixture of soil and saliva were tested according the methods of Weller and Cook, 1983 and El-Sohaibani, 1999 for bacteria and Umechuruba and Nwachukwa (1997) for fungi.