The preparation of honey samples for investigating the presence of C. botulinum by using conventional microbiological methods and PCR method was conducted by enrichment, centrifugation and membrane filtration9. Seeding on Zeissler blood and egg yolk agar (EYA) was performed from prepared honey samples after which cultural, microscopic and biochemical identification of isolated bacteria cultures was completed. The conventional method, ISO 15213:2003 was applied to investigate the presence of C. botulinum in uncontaminated and deliberately contaminated honey samples. The PCR method of detecting C. botulinum was used to detect DNA of C. botulinum in prepared honey samples, described by Lindström and Lindström’s collaborators9. Twenty replicates were processed from each honey sample. The most probable number method (MPN) was utilized to determine the C. botulinum number of spores in honey samples