Thiobarbituric acid reactive substances (TBARS) formed due to
lipid peroxidation during storage were determined using the
method of Alasnier, Meynier, Viau, and Gandmer (2000). A 4-g
portion of sample was blended with 16 ml of trichloroacetic acid
(5 g/100 ml) and BHT (10 mg BHT/g of lipid). It was then filtered
through Whatman filter (No. 4). Equal volumes of the filtrate and
0.02 mol/L thiobarbituric acid solution were heated in a boiling
water bath for 30 min, cooled and the absorbance was measured at
532 nm. The amount of TBARSwas expressed as mg malonaldehyde
per kg of sample.