This investigation
proposed to test previously isolated bioactive compounds from B. rotunda and M. fragrans if they possessed
anti- H. pylori activity. Primary cultures of H. pylori from local hospital patients in Thailand were used in the
investigation. In vitro anti- H. pylori testing had been performed with pinostrobin and red oil from roots of
B. rotunda, and dihydroguaiaretic acid from arils of M. fragrans. Clarithromycin (MIC 120 μg/mL) was used
as a positive control. All three compounds showed positive clear zone in agar diffusion test at p