To examine the effect of 2,4-D on somatic embryo induction,
proliferated callus was placed on a solid MS medium supplemented
with different concentrations of 2,4-D (0 mg/L, 0.5 mg/L, 1 mg/L).
Ten clumps of embryogenic callus (about 5 mm in diameter) were
cultured on petri dishes containing 40 mL of medium and the
experiment repeated three times. All cultures were incubated at
25 ? C in the dark. The frequency of somatic embryo production was
examined after 6 wk of culture by counting cultured embryogenic
calluses that formed somatic embryos