By the end of the same experiment (at 281 dpi), there were 30 seedlings apparently healthy. In order to further explore symptom development of these seedlings, they were transplanted to pots and grown in a greenhouse at 18 ± 5 °C with relative humidity of 55%–60%. Before this, seven of these seedlings were randomly chosen and processed as described above to estimate cfu of F. circinatum. The remaining 23 seedlings were monitored until 458 dpi. As seedlings died, plant parts (roots, stems and tips) were cut in transversal sections, plated on SFA and checked for presence of F. circinatum. Two plants survived at the end of this period.