Culture of the plant material
Sterile seeds ready for culture, were inoculated into
petri dishes using a sterile forceps. The inoculated seeds
germinated, and it was observed that seedlings with
fragmented leaves formed within 21 days (Figures 2 and
3). Strong sprouts taken from well-developed plants were
sent to the laboratory, and were fist trimmed to a smaller
size and then washed in soapy water. Their surface
was sterilized by means of agitation in 20% sodium
hypochloride solution for 20 minutes. This procedure
was performed in a sterile container, and 1-1.5 mm-long
tips of the sprouts taken from the last washing solution
were inoculated into the medium, such that the tips of the
fist shoots to develop were used for rooting.