Laccase (EC 1.10.3.2, Lac), secreted by most of the WRF, is one of the most important lignin oxidation enzymes that can non-specifically degrade many phenolic and non-phenolic compounds, such as lignin and many environmental pollutants (including synthetic dyes, toxic substances found in industrial effluents, and herbicides and pesticides in the environment) (Rodríguez Couto and Toca Herrera, 2006 and Rodgers et al., 2009). However, in terms of the practical applications of laccases to decolorize and detoxify dyes, such as MG and MG-containing wastewater, there are still many difficulties to overcome. Industrial waste generally contains high levels of salt, metal ions and organic compounds, which could cause laccase denaturation (Grassi et al., 2011). Therefore, there is an urgent need for a laccase with tolerance to organic solvents, high temperatures and ionic concentrations, functionality over a wide pH range.