2.2.2. Fatty acid composition
The fatty acid compositions were analyzed using the American
Oil Chemists’ Society (AOCS) Ce 2-66 method by employing a capillary
gas chromatograph (CGC AGILENT 6850 Series GC system)
with an auto sampler and flame ionization detector (FID) (AOCS,
2009). The fatty acid methyl esters were separated according to
the method of Hartman and Lago (1973) using a capillary column
(DB-23 AGILENT; 50% cyanopropylmethylpolysiloxane, 60 m long,
0.25 mm internal diameter, 0.25 lm thick film). The analysis conditions
were as follows: oven temperature: 110 C/5 min, 110–
215 C (5C/min), 215 C/24 min; detector temperature: 280 C;
injector temperature: 250 C; carrier gas: helium, split ratio 1:50;
injection volume: 1 lL. The qualitative composition was determined
by comparing the peak retention times measured for the
samples with those of the respective standards for the fatty acids.
The iodine values were calculated based on the fatty acid compositions,
according to the AOCS official method (Cd 1c-85). The analyses
were performed in triplicate.