The egg yolk hydrolysis can be one of indicator to determine the characteristic of X. oryzae species. This test is based on the observation that the enzyme lecithinase can break down the phospholipid emulsion of egg yolk, liberating a turbid zone of free fats around the colonies. The egg emulsion in the agar provides the lecithin to be degraded by the enzyme. A given isolates that contain lecithinase break down the egg yolk and cause clear zones around the colonies [16]. Sometimes an opaque zone of precipitation may form due to lipase activity around that clear zone. However, out of 15 Xoo isolates, only four isolates (Xoo-9, Xoo-10, XOR and Xoo-19) managed to hydrolyze the egg yolk (Table 1), the rest unable to degrade the protein due to an absence of the enzymes.