The aim of the present work was to examine two
probiotic Lactobacillus strains of dairy origin for their
performance as adjuncts in the production of set type
yoghurt. The strains were selected on the basis of their
probiotic potential (Maragkoudakis et al., 2005). After
the initial yoghurt trials, strain L. paracasei subsp.
tolerans ACA-DC 4037 emerged as the best candidate
for probiotic yoghurt production, mainly because of the
very good sensory properties of the manufactured
yoghurt. L. paracasei subsp. tolerans ACA-DC 4037
has been isolated from traditional Greek Kasseri cheese,
and thus can be considered safe. In addition, it has been
found to be resistant in conditions simulating the GI
tract, able to induce strong pro-inflammatory reaction in
PBMC cells and to inhibit the attachment of E. coli and
S. typhimurium to Caco-2 cells (Maragkoudakis et al.,
2005). With this strain as an adjunct, high-quality
yoghurt was produced with population levels satisfying
the international recommendations and guidelines for
probiotic and starter culture bacteria. The physicochemical
properties of this probiotic yoghurt did not differ
from the control and were acceptable by the standards
set by the collaborating dairy company. All attempts to
use an inoculum different from curdled milk were not
successful. The main problems were the long fermentation
period required to achieve pH 4.6 and the
subsequent poor sensory properties of the yoghurt.
Although further investigations are required, especiallyfor the use of microencapsulated bacteria in fermented
milks, the scope of this work has been achieved,
producing high-quality yoghurt with a potential probiotic
strain. Subsequent in vivo trials and clinical
studies should be performed before such a product can
be marketed, in order to verify any potential health
benefits claimed.
The aim of the present work was to examine two
probiotic Lactobacillus strains of dairy origin for their
performance as adjuncts in the production of set type
yoghurt. The strains were selected on the basis of their
probiotic potential (Maragkoudakis et al., 2005). After
the initial yoghurt trials, strain L. paracasei subsp.
tolerans ACA-DC 4037 emerged as the best candidate
for probiotic yoghurt production, mainly because of the
very good sensory properties of the manufactured
yoghurt. L. paracasei subsp. tolerans ACA-DC 4037
has been isolated from traditional Greek Kasseri cheese,
and thus can be considered safe. In addition, it has been
found to be resistant in conditions simulating the GI
tract, able to induce strong pro-inflammatory reaction in
PBMC cells and to inhibit the attachment of E. coli and
S. typhimurium to Caco-2 cells (Maragkoudakis et al.,
2005). With this strain as an adjunct, high-quality
yoghurt was produced with population levels satisfying
the international recommendations and guidelines for
probiotic and starter culture bacteria. The physicochemical
properties of this probiotic yoghurt did not differ
from the control and were acceptable by the standards
set by the collaborating dairy company. All attempts to
use an inoculum different from curdled milk were not
successful. The main problems were the long fermentation
period required to achieve pH 4.6 and the
subsequent poor sensory properties of the yoghurt.
Although further investigations are required, especiallyfor the use of microencapsulated bacteria in fermented
milks, the scope of this work has been achieved,
producing high-quality yoghurt with a potential probiotic
strain. Subsequent in vivo trials and clinical
studies should be performed before such a product can
be marketed, in order to verify any potential health
benefits claimed.
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