This study aims to investigate the changes ofthe bovine, camel, and caprine milk proteins after freezing, pasteurization (62 °C, 30 min), and spray drying byproteomic techniques,filter-aided sample preparation (FASP) and dimethyl labeling followed by liquid chroma-tography–tandem mass spectrometry (LC–MS/MS). A total of 129, 125, and 74 proteins were quantified in bo-vine, camel, and caprine milk sera, respectively.