INTRODUCTION AND BACKGROUND
Tomato (LycopersicmesculentumL.spp) is the most important vegetable crop cultivated worldwide with a high nutritive
value, India the second top producers of tomatoes with an annual production of 17,500,000 tonnes (FAOSTAT, 2012).
Tomato plants are highly suspectible to various diseases like viral, bacterial, fungal and nematode disease. Early blight
(EB) is the most common disease of tomato caused by Alternariasolani, affects the yield and reduces fruit quality. EB
is a serious problem in warm and humid regions [1] and also in semiarid areas. EB reduces the photosynthetic area, and
defoliates the lower parts of leaves, it forms brown spots on leaf, fruits and stems, if uncontrolled early the entire plant
collapses. An attempt was made to genetically modify the crop with antimicrobial peptide gene (Ace-AMP1) which
brings aboutcytoplasmatic membrane disruption [2] [3] and also can inhibit several cellular processes such as nucleic
acid and protein synthesis, enzymatic activity and cell wall synthesis [4] [5] [6] [7] [8. Transgenic plants were
developed for resistance towards early blight disease. There are several techniques employed to screen transgenic
plants resistant towards a disease. The normal way of screening for disease is by field and greenhouse screening which
is time consuming, affected by various external environmental factors such as temperature, humidity and influence of
other pathogens in the study. These methods require challenging of plants with pathogens and there is a possibility of
contaminating the atmosphere thus resulting in spread of disease to all the healthy plants. In view of all the most
efficient and simpler attempt is by the detached leaf bioassay, with correct aged leaf in a laboratory condition, thus
INTRODUCTION AND BACKGROUNDTomato (LycopersicmesculentumL.spp) is the most important vegetable crop cultivated worldwide with a high nutritivevalue, India the second top producers of tomatoes with an annual production of 17,500,000 tonnes (FAOSTAT, 2012).Tomato plants are highly suspectible to various diseases like viral, bacterial, fungal and nematode disease. Early blight(EB) is the most common disease of tomato caused by Alternariasolani, affects the yield and reduces fruit quality. EBis a serious problem in warm and humid regions [1] and also in semiarid areas. EB reduces the photosynthetic area, anddefoliates the lower parts of leaves, it forms brown spots on leaf, fruits and stems, if uncontrolled early the entire plantcollapses. An attempt was made to genetically modify the crop with antimicrobial peptide gene (Ace-AMP1) whichbrings aboutcytoplasmatic membrane disruption [2] [3] and also can inhibit several cellular processes such as nucleicacid and protein synthesis, enzymatic activity and cell wall synthesis [4] [5] [6] [7] [8. Transgenic plants weredeveloped for resistance towards early blight disease. There are several techniques employed to screen transgenicplants resistant towards a disease. The normal way of screening for disease is by field and greenhouse screening whichis time consuming, affected by various external environmental factors such as temperature, humidity and influence ofother pathogens in the study. These methods require challenging of plants with pathogens and there is a possibility ofcontaminating the atmosphere thus resulting in spread of disease to all the healthy plants. In view of all the mostefficient and simpler attempt is by the detached leaf bioassay, with correct aged leaf in a laboratory condition, thus
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