SdFFF
Analysis of the final samples was done using a Postnova S101 particle fractionator
(Postnova Analytics, Salt Lake City, UT). The injected sample volume
was 100 μlusing 0.1% FL-70 as the carrier fluid at a rate of 2 ml/min. The initial speed ofcentrifugation was 1800 rpm and the final speed was 200 rpm. SdFFF run time was typically 90 min. Detection was achieved with a light scattering detector
(Brookhaven, Holtsville, NY) at a 90° angle (690 nm laser). A companion paper
[19] provides further details of the SdFFF methodology.